Aplikasi Enzim Laktase pada Produksi Yogurt Bebas Laktosa dan Pengaruhnya terhadap Karakteristik Produk
Keywords:
β-galactosidase, Lactose-free Yogurt, Lactose Intolerance, YogurtAbstract
Yogurt merupakan produk susu fermentasi yang masih mengandung laktosa sehingga kurang sesuai dikonsumsi oleh individu dengan intoleransi laktosa. Penambahan enzim laktase (β-galaktosidase) menjadi salah satu pendekatan yang efektif untuk menghasilkan yogurt bebas laktosa tanpa mengurangi kualitas produk. Artikel ini bertujuan mengkaji aplikasi enzim laktase dalam produksi yogurt bebas laktosa serta pengaruhnya terhadap karakteristik produk. Kajian ini disusun menggunakan metode narrative literature review terhadap berbagai publikasi ilmiah yang relevan. Hasil kajian menunjukkan bahwa enzim laktase secara efektif menghidrolisis laktosa menjadi glukosa dan galaktosa sehingga menghasilkan yogurt dengan kadar laktosa sangat rendah hingga tidak terdeteksi. Hidrolisis laktosa juga mendukung aktivitas bakteri asam laktat selama fermentasi sehingga menghasilkan pH akhir berkisar 4,1–4,9. Penambahan enzim laktase tidak menurunkan kualitas tekstur maupun karakteristik sensori yogurt, bahkan melalui formulasi yang tepat dapat meningkatkan viskositas, stabilitas gel, rasa manis alami, dan penerimaan konsumen. Secara keseluruhan, aplikasi enzim laktase merupakan strategi yang efektif dalam menghasilkan yogurt bebas laktosa dengan karakteristik fisikokimia, tekstur, dan sensori yang setara atau lebih baik dibandingkan yogurt konvensional, sehingga berpotensi menjadi alternatif pangan fungsional bagi penderita intoleransi laktosa.
Downloads
References
Abdel Wahab, W. A., Ahmed, S. A., Kholif, A. M. M., Abd El Ghani, S., & Wehaidy, H. R. (2021). Rice straw and orange peel wastes as cheap and eco-friendly substrates: A new approach in β-galactosidase (lactase) enzyme production by the new isolate L. paracasei MK852178 to produce low-lactose yogurt for lactose-intolerant people. Waste Management, 131, 403–411. https://doi.org/10.1016/j.wasman.2021.06.028
Abdel Wahab, W. A., Ahmed, S. A., Kholif, A. M. M., El Ghani, S. A., & Wehaidy, H. R. (2024). Low-lactose yoghurt production using β-galactosidase: An integrated study for the enzyme and its application. International Dairy Journal, 151, 105864. https://doi.org/10.1016/j.idairyj.2023.105864
Andrani, M., Dall’Olio, E., De Rensis, F., Tummaruk, P., & Saleri, R. (2024). Bioactive Peptides in Dairy Milk: Highlighting the Role of Melatonin. Biomolecules, 14(8), 934. https://doi.org/10.3390/biom14080934
Ayivi, R. D., & Ibrahim, S. A. (2022). Lactic acid bacteria: An essential probiotic and starter culture for the production of yoghurt. International Journal of Food Science & Technology, 57(11), 7008–7025. https://doi.org/10.1111/ijfs.16076
Bi̇çer, Y., Turkal, G., Telli̇, A. E., Bayır, T., & Sert, D. (2025). Comparative study of the outcome of yogurt-type fermentation of lactose-containing and lactose-free cow and sheep milk. Small Ruminant Research, 252, 107583. https://doi.org/10.1016/j.smallrumres.2025.107583
Brishti, M. R., Venkatraman, G., Baba, A. S. B. H., Yajit, N. L. M., & Karsani, S. A. (2026). Natural Bioactive Compounds Enriched Functional Yogurt: Impact on the Probiotic Bacteria and Its Potential Health Benefits. Probiotics and Antimicrobial Proteins, 18(1), 1360–1377. https://doi.org/10.1007/s12602-025-10461-1
Catanzaro, R., Sciuto, M., & Marotta, F. (2021). Lactose Intolerance—Old and New Knowledge on Pathophysiological Mechanisms, Diagnosis, and Treatment. SN Comprehensive Clinical Medicine, 3(2), 499–509. https://doi.org/10.1007/s42399-021-00792-9
Chebykin, Y. S., Musin, E. V., Kim, A. L., & Tikhonenko, S. A. (2025). Lactose removal in dairy products using immobilized on glass plates polyelectrolyte microcapsules with encapsulated β-galactosidase. Innovative Food Science & Emerging Technologies, 105, 104180. https://doi.org/10.1016/j.ifset.2025.104180
Costa, J. B., Nascimento, L. G. L., Martins, E., & Carvalho, A. F. D. (2024). Immobilization of the β-galactosidase enzyme by encapsulation in polymeric matrices for application in the dairy industry. Journal of Dairy Science, 107(11), 9100–9109. https://doi.org/10.3168/jds.2024-24892
Dantas, A., Verruck, S., Canella, M. H. M., Hernandez, E., & Prudencio, E. S. (2021). Encapsulated Bifidobacterium BB-12 addition in a concentrated lactose-free yogurt: Its survival during storage and effects on the product’s properties. Food Research International, 150, 110742. https://doi.org/10.1016/j.foodres.2021.110742
Dhar, H., Verma, S., Dogra, S., Katoch, S., Vij, R., Singh, G., & Sharma, M. (2023). Functional attributes of bioactive peptides of bovine milk origin and application of in silico approaches for peptide prediction and functional annotations. Critical Reviews in Food Science and Nutrition, 1–23. https://doi.org/10.1080/10408398.2023.2212803
Facioni, M. S., Raspini, B., Pivari, F., Dogliotti, E., & Cena, H. (2020). Nutritional management of lactose intolerance: The importance of diet and food labelling. Journal of Translational Medicine, 18(1), 260. https://doi.org/10.1186/s12967-020-02429-2
Li, X., Wang, L., Guan, B., Xu, C., Hu, Y., Han, R., Li, X., Ni, Y., Zeng, J., & Amirzada, M. I. (2025). Biochemical characterization of a β-galactosidase from Lactiplantibacillus plantarum YLBGNL-S7 and utilization of its permeabilized whole-cell catalyst for in situ galactooligosaccharide synthesis during yogurt making. LWT, 215, 117234. https://doi.org/10.1016/j.lwt.2024.117234
Miao, M., Li, S., Yang, S., Yan, Q., Xiang, Z., & Jiang, Z. (2024). Engineering the β-galactosidase from Aspergillus oryzae for making lactose-free and no-sugar-added yogurt. Journal of Dairy Science, 107(9), 6602–6613. https://doi.org/10.3168/jds.2023-24310
Pinto, S. S., Fritzen-Freire, C. B., Dias, C. O., & Amboni, R. D. M. C. (2019). A potential technological application of probiotic microcapsules in lactose-free Greek-style yoghurt. International Dairy Journal, 97, 131–138. https://doi.org/10.1016/j.idairyj.2019.05.009
Skryplonek, K., Henriques, M., Gomes, D., Viegas, J., Fonseca, C., Pereira, C., Dmytrów, I., & Mituniewicz-Małek, A. (2019). Characteristics of lactose-free frozen yogurt with κ-carrageenan and corn starch as stabilizers. Journal of Dairy Science, 102(9), 7838–7848. https://doi.org/10.3168/jds.2019-16556
Tagliazucchi, D., Martini, S., & Solieri, L. (2019). Bioprospecting for Bioactive Peptide Production by Lactic Acid Bacteria Isolated from Fermented Dairy Food. Fermentation, 5(4), 96. https://doi.org/10.3390/fermentation5040096
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Nia Ulfa Madani, Yuliatin Hasfiani

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.





