SAINTEKES: Jurnal Sains, Teknologi Dan Kesehatan https://ejournal.itka.ac.id/index.php/saintekes <p><strong>SAINTEKES : Jurnal Sains, Teknologi dan Kesehatan</strong> is a peer-review journal that could be access to the public, published by LPPM Institut Teknologi Dan Kesehatan Aspirasi. This journal is published quarterly, every <strong>January</strong>, <strong>April</strong>, <strong>July</strong> and <strong>October</strong> with online version of <a href="https://issn.brin.go.id/terbit/detail/20220908451679245" target="_blank" rel="noopener"><strong>E-ISSN: 2963-0959</strong></a>. <strong>SAINTEKES</strong> provides a platform for researchers, academics, professionals, practitioners and students to embed and share knowledge in the form of empirical and theoretical research papers, case studies, literature reviews and book reviews related to science, technology and health research. <strong>SAINTEKES</strong> welcomes and recognizes high quality theoretical and empirical research papers, case studies, review papers, literature reviews, book reviews, conceptual frameworks, analysis and simulation models, technical notes related to research from researchers, academics, professionals, practitioners, and students.</p> LPPM Institut Teknologi Dan Kesehatan Aspirasi en-US SAINTEKES: Jurnal Sains, Teknologi Dan Kesehatan 2963-0959 Etnomedisin Tumbuhan Obat untuk Mengobati Penyakit Hipertensi oleh Pengobat Tradisional di Kecamatan Ambalau Kabupaten Buru Selatan https://ejournal.itka.ac.id/index.php/saintekes/article/view/170 <p><em>Hypertension is a condition in which blood pressure rises abnormally and continuously during several blood pressure checks.</em> <em>Traditional medicine is still used by the people of Indonesia in the current era of globalization, even though modern medicine has been applied.</em> <em>The purpose of this study was to study ethnomedicine medicinal plants for treating hypertension by traditional healers in Ambalau District, South Buru Regency.</em> <em>This type of research is descriptive qualitative with an observational exploratory design.</em> <em>Data was collected using in-depth interview techniques with 35 informants.</em> <em>Obtained data were analyzed qualitatively.</em> <em>The results showed that the types of plants used in the treatment of hypertension include: fragrant pandan, African leaves, lima leaves, order, soursop and soursop.</em> <em>This indicates that the healers in the Ambalau sub-district have the same knowledge in utilizing medicinal plants to treat hypertension.</em> <em>Apart from that, there are also similarities in the parts that are widely used, namely herbs and leaves</em><em>.</em></p> Muhammad Fitrah Loilatu Abdul M Ukratalo Dodikrisno E Manery Victory O Pangemanan Copyright (c) 2024 Muhammad Fitrah Loilatu, Abdul M Ukratalo, Dodikrisno E Manery, Victory O Pangemanan https://creativecommons.org/licenses/by-sa/4.0 2024-04-30 2024-04-30 3 2 107 118 10.55681/saintekes.v3i2.170 Analisis Kandungan DNA Babi pada Tempat Penggilingan Daging di Pasar-Pasar Kelurahan Cempaka Putih dan Tinjauannya Menurut Pandangan Islam https://ejournal.itka.ac.id/index.php/saintekes/article/view/179 <p>Cemaran daging babi masih menjadi salah satu permasalahan dalam berlangsungnya kegiatan perekonomian pengadaan daging di masyarakat, salah satunya ada pada produk daging giling. Tidak seluruh tempat penggilingan daging di pasar-pasar dapat menjamin bahwa daging yang digiling merupakan daging halal tanpa adanya kontaminasi babi. Tujuan penelitian ini adalah menganalisis cemaran DNA babi pada 5 sampel daging giling yang diperoleh dari tempat penggilingan daging di pasar-pasar Kelurahan Cempaka Putih. Proses analisis DNA dilakukan dengan menggunakan metode RT-PCR (<em>Real-Time Polymerase Chain Reaction). </em>Kelima sampel akan dilakukan isolasi DNA menggunakan <em>Mericon Food Kit</em> lalu dianalisis kemurnian dan konsentrasinya dengan menggunakan alat sprektrofotometer, selanjutnya sampel akan dilakukan proses RT-PCR dengan menggunakan <em>Mericon Pig Kit.</em> Analisis DNA sampel menunjukkan hasil negatif diseluruh sampel daging giling dan kontrol negatif ditandai dengan tidak adanya kenaikan garis kurva amplifikasi pada chanel FAM, sementara kontrol positif DNA babi menunjukkan hasil positif dengan adanya kenaikan nilai Cq (<em>quantification cycle)</em> pada kurva amplifikasi chanel FAM. Hal ini menandakan proses RT-PCR berlangsung dengan baik dan 5 sampel daging giling tidak mengandung DNA babi.</p> Anggun Widiyaningsih Yulia Suciati Endy Muhammad Astiwara Copyright (c) 2024 Anggun Widiyaningsih, Yulia Suciati, Endy Muhammad Astiwara https://creativecommons.org/licenses/by-sa/4.0 2024-04-30 2024-04-30 3 2 119 126 10.55681/saintekes.v3i2.179 Pengaruh Sifat Biji Jarak sebagai Bahan Bakar Alternatif Penganti Minyak Tanah untuk Pengeringan Tembakau Virgina Lombok https://ejournal.itka.ac.id/index.php/saintekes/article/view/320 <p>Virginia tobacco is the largest agro-industry component in West Nusa Tenggara (NTB). The lack of subsidized kerosene quotas for tobacco extraction, even the central government decided that in 2018 the sub-oil kerosene quota will be abolished, brings its own problems. Based on this problem, research was carried out with the title how to characterize castor beans as an alternative fuel to replace kerosene as a substitute for tobacco extraction fuel with the aim of proving that castor beans can be used as an alternative fuel for drying tobacco. process without reducing the quality and economy of tobacco. This research was carried out experimentally. From the research results it can be concluded that castor bean fuel can be used as a fuel substitute for kerosene for tobacco production as proven by the results of the characteristic test, namely the calorific value produced in this research has met the national standard (SNI), namely 5288.844 cal/gr based on the standard. which meets American standards, namely 4000-6500 (Source: Hendra, 1999 in Sunyata and Wulur, 2008). economic value aspect of fuel consumption / 1 kg of dry castor beans consumes 6.7 kg of castor beans at a price of Rp. 6,700 lower than kerosene, LPG and bioethanol. Variable standard drying temperature with a drying rate of 1 m/s produces tobacco compost with the best quality color and aroma.</p> Ahmad Multazam Khairul Rizal Copyright (c) 2024 Ahmad Multazam, Khairul Rizal https://creativecommons.org/licenses/by-sa/4.0 2024-04-30 2024-04-30 3 2 127 134 10.55681/saintekes.v3i2.320 Kajian Sintesis Asam Lemak Hidroksamat Menggunakan Minyak Goreng Bekas https://ejournal.itka.ac.id/index.php/saintekes/article/view/326 <p><em>Fatty Hydroxamic Acids (FHA) has been successfully synthesized from used cooking oil. The purpose of this research was the enzymatic synthesis of fatty hydroxamic acids from used cooking oil and determine the optimum conditions, which includes three stages of processing, namely the oil preparation stage, the synthesis optimization stage and the characterization stage. At the oil preparation stage, the yield of used cooking oil was 29%. At the optimization stage, the optimum conditions for the synthesis of fatty hydroxamic acids from used cooking oil were at a temperature of 35<sup>o</sup>C for 25 hours with a ratio of lipase (gram) : hydroxylamine (mmol) equal to 1:500 and the ratio of lipase (gram) : crude bran rice (gram) was equal to 1:75. The number of hydroxamic acid groups in 1 gram of dry sample of fatty hydroxamic acid was 3,16 mmol. Based on the results of the analysis of the color test with Cu (II) and Fe (III), a complex color that is typical for the two metals which obtained with fatty hydroxamic acids, they were green and dark red. Whereas, from the FTIR analysis, the spectrum of the functional groups of hydroxamic acid was obtained.</em></p> Muhsinun Muhsinun Copyright (c) 2024 Muhsinun https://creativecommons.org/licenses/by-sa/4.0 2024-04-30 2024-04-30 3 2 135 141 10.55681/saintekes.v3i2.326 Pengaruh Pemberian Ekstrak Bengkuang (Pachyrhizus Erosus) sebagai Antioksidan terhadap Kadar Kolesteroltinggi pada Mencit (Mus Musculus) https://ejournal.itka.ac.id/index.php/saintekes/article/view/327 <p><em>High cholesterol levels in the blood are a serious problem because they are a risk factor for various non-communicable diseases such as heart disease, stroke and diabetes mellitus. Empirically, the secondary metabolic contents of jicama such as saponins&amp; flavonoids have their respective properties. Saponin functions to bind cholesterol with bile acids so that it can reduce cholesterol levels. The results of the antioxidant activity test, the IC50 value of jicama extract is 115.20 mg/mL, which indicates that the antioxidant activity of jicama extract is classified as a moderate antioxidant. The results of measuring cholesterol levels after treatment showed that there was a decrease in cholesterol levels after giving jicama extract treatment in each group, seen in treatment 1 (P1) at 113.4 with a difference of 2.6 from cholesterol before administration, treatment 2 (P2) at 110.8 with a difference of 4.6 of cholesterol before administration, treatment 3 (P3) was 110.6 with a difference of 7.6 from cholesterol before administration of jicama extract, positive control (K+) was 108.4 with a difference of 30.2 from cholesterol before administration, negative control (K-) was 122.6 with a difference of 0.2 antioxidants from purple cabbage, namely 115.20 μg/mL. The antioxidant activity of purple cabbage is in the very strong category and vitamin C is in the very strong category.</em></p> Roushandy Asri Fardani Ika Nurfajri Mentari Eka Fitria Copyright (c) 2024 Roushandy Asri Fardani, Ika Nurfajri Mentari, Eka Fitria https://creativecommons.org/licenses/by-sa/4.0 2024-04-30 2024-04-30 3 2 142 146 10.55681/saintekes.v3i2.327